Schedule:
Every Wednesday from 3/31-4/28
Time: from 8-11 am MDT
(7-10 am PDT, 9-12 CDT,
10-1 EDT, 16-19 CET) |
| 3/31: | Introduction
- AUC and Hydrodynamics basics
- UltraScan overview
- UltraScan-in-a-Box setup
- Accessing the system via ssh, vnc
- Questions and Answers
- Workshop Recording Video
|
| 4/7: | UltraScan basics and Sed. Veloc. Analysis I
- UltraScan LIMS, uploading and editing
- Understanding Noise in Data
- Finite Element Modeling Basics
- Model Dependent vs. Model Independent Analysis
- Sed. Veloc. Analysis Workflow
- Boundary Condition fitting
- Reversible interactions
- 2-dimensional Spectrum Analysis + Demo
- Questions and Answers
- Workshop Recording Video
|
| 4/14: | Sed. Veloc. Analysis II
- Parametrically Constrained Spectrum Analysis + Demo
- Genetic Algorithms + Demo
- Monte Carlo analysis
- Global Analysis
- van Holde - Weischet Analysis
- Case Studies:
- Reversible Association
- Aggregation
- Hetero-Interactions
- Molar Mass, Size and Shape analysis
- Questions and Answers
- Workshop Recording Video
|
| 4/21: | Experimental Design
- Cells, Centerpieces, Rotors, Optics, loading volume, # of scans
- Rotor Speed and Sed. Velocity Simulation
- Sed. Equilibrium vs. Sed. Velocity
- What AUC can and cannot tell you
- How to optimize your experimental design
- Analysis Strategies
- Questions and Answers
- Workshop Recording Video
|
| 4/28: | Advanced Concepts
- Concepts in multi-wavelength AUC experiments
- Application examples:
- AAV
- Lipid Nanoparticles
- Protein-Nucleic Acid Interactions
- Discrete Model Genetic Algorithms + Demo
- Custom Grid Analysis and Applications + Demo
- Partial Specific Volume Distributions and Density
- Instrument Calibrations
- Questions and Answers
- Workshop Recording Video
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