19th Annual UltraScan Analytical Ultracentrifugation Workshop 2021

Session 1: March 31st, 2021 (8-11 am, MDT)
Session 2: April 7th, 2021 (8-11 am, MDT)
Session 3: April 14th, 2021 (8-11 am, MDT)
Session 4: April 21st, 2021 (8-11 am, MDT)
Session 5: April 28th, 2021 (8-11 am, MDT)
-- sponsored by the Canadian Center for Hydrodynamics in Lethbridge, Alberta.


Solution Studies of Biological Macromolecules:
From Experimental Design to Data Analysis - UltraScan-III Workshop

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Instructor: Dr. Borries Demeler, Canada 150 Research Chair for Biophysics, University of Lethbridge
Schedule:
Every Wednesday from 3/31-4/28
Time: from 8-11 am MDT
(7-10 am PDT, 9-12 CDT, 10-1 EDT, 16-19 CET)
3/31:Introduction
  • AUC and Hydrodynamics basics
  • UltraScan overview
  • UltraScan-in-a-Box setup
  • Accessing the system via ssh, vnc
  • Questions and Answers
  • Workshop Recording Video

4/7:UltraScan basics and Sed. Veloc. Analysis I
  • UltraScan LIMS, uploading and editing
  • Understanding Noise in Data
  • Finite Element Modeling Basics
  • Model Dependent vs. Model Independent Analysis
  • Sed. Veloc. Analysis Workflow
  • Boundary Condition fitting
  • Reversible interactions
  • 2-dimensional Spectrum Analysis + Demo
  • Questions and Answers
  • Workshop Recording Video

4/14:Sed. Veloc. Analysis II
  • Parametrically Constrained Spectrum Analysis + Demo
  • Genetic Algorithms + Demo
  • Monte Carlo analysis
  • Global Analysis
  • van Holde - Weischet Analysis
  • Case Studies:
    • Reversible Association
    • Aggregation
    • Hetero-Interactions
    • Molar Mass, Size and Shape analysis
  • Questions and Answers
  • Workshop Recording Video

4/21:Experimental Design
  • Cells, Centerpieces, Rotors, Optics, loading volume, # of scans
  • Rotor Speed and Sed. Velocity Simulation
  • Sed. Equilibrium vs. Sed. Velocity
  • What AUC can and cannot tell you
  • How to optimize your experimental design
  • Analysis Strategies
  • Questions and Answers
  • Workshop Recording Video

4/28:Advanced Concepts
  • Concepts in multi-wavelength AUC experiments
  • Application examples:
    • AAV
    • Lipid Nanoparticles
    • Protein-Nucleic Acid Interactions
  • Discrete Model Genetic Algorithms + Demo
  • Custom Grid Analysis and Applications + Demo
  • Partial Specific Volume Distributions and Density
  • Instrument Calibrations
  • Questions and Answers
  • Workshop Recording Video

Details: