21st Annual UltraScan Analytical Ultracentrifugation Workshop 2024

August 14-16, 2024, in Missoula, Montana, USA.

Sponsored by:
Beckman Coulter, AUC Solutions and the Northern Rockies Center for Hydrodynamics at the University of Montana


US-SOMO Hydrodynamic Modelling

UltraScan Solution Modeler Hydrodynamic Modelling Workshop

Presenters: Saeed Mortezazadeh, University of Lethbridge, Canada (in-person), Emre Brookes, University of Montana, USA (remote), and Mattia Rocco, Retired, Ospedale Policlinico San Martino, Genova, Italy (remote)

Description

UltraScan Solution Modeler (US-SOMO)[1,2] processes atomic and lower-resolution bead model representations of biological and other macromolecules to compute various hydrodynamic parameters, such as the sedimentation and diffusion coefficients, relaxation times and intrinsic viscosity. This allows the researcher to validate structural models against experimental data. The tools available in US-SOMO have been shown to provide the best known computations of hydrodynamic parameters from experiment [3]. The US-SOMO hydrodynamics workshop will consist of three sessions. In the first session, attendees will be given an introduction to computational hydrodynamics and techniques. In the second session, users will utilize US-SOMO to compute hydrodynamics parameters from structures and to use automated batch processing to compute over multiple structures.

[1] Brookes, E. and Rocco, M., 2018. Recent advances in the UltraScan SOlution MOdeller (US-SOMO) hydrodynamic and small-angle scattering data analysis and simulation suite. European Biophysics Journal, 47(7), pp.855-864. doi: 10.1007/s00249-018-1296-0.

[2] Rocco, M., Brookes, E. & Byron, O. US-SOMO: Methods for Construction and Hydration of Macromolecular Hydrodynamic Models. In: Encyclopedia of Biophysics, Roberts, G. & Watts, A., European Biophysical Societies (eds). Springer, Berlin, Heidelberg (2021). doi: 10.1007/978-3-642-35943-9_292-1

[3] Rocco M, Byron O., 2015, "Hydrodynamic Modeling and Its Application in AUC.", Methods Enzymol. 2015;562:81-108. doi: 10.1016/bs.mie.2015.04.010.